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目的:研究体外培养的人胎脑源性神经前体细胞的致瘤性。方法:将人胎脑源神经前体细胞体外培养至第1、25、40、60代,且每代细胞分别制备成神经球及单个细胞混悬液两种制剂,并取293T细胞作为阳性对照,共9组,每组5只,分别皮下接种于4~8周龄的BALB/C裸鼠,接种后饲养6个月,定期观察裸鼠精神状态、饮食、排便、以及接种局部有无出现结节或肿块,接种6个月后处死裸鼠,对接种局部及内脏进行组织病理切片及HE染色。结果:将人胎脑源性神经前体细胞接种于裸鼠皮下6个月未见肿瘤形成,且未见其他异常组织形成;而阳性对照组293T细胞接种于裸鼠皮下1个月后可见明显肿瘤形成。结论:人胎脑源神经前体细胞对裸鼠不具有体内致瘤性。
Objective: To study the tumorigenicity of human fetal brain-derived neural precursor cells cultured in vitro. Methods: Human fetal brain-derived neural progenitor cells were cultured in vitro to the first, 25th, 40th, 60th and 60th generations, and each generation of cells were prepared into neurospheres and single cell suspension respectively, and 293T cells were used as positive control , A total of 9 groups, 5 in each group, were subcutaneously inoculated 4 to 8 weeks old BALB / C nude mice, after inoculation 6 months, regular observation of the nude mice mental state, diet, defecation, and inoculation of the local presence of Nodules or lumps. After 6 months of inoculation, the nude mice were sacrificed and the pathological sections of the inoculated and visceral organs were stained and HE stained. Results: Human fetal brain-derived neural progenitor cells were inoculated subcutaneously in nude mice for 6 months without tumor formation, and no other abnormal tissue formation was observed. Positive control 293T cells were inoculated subcutaneously in nude mice for 1 month Tumor formation. Conclusion: Human fetal neural precursor cells do not have in vivo tumorigenicity in nude mice.