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目的研究人肝细胞癌中p14ARF蛋白的表达缺失情况及其与临床病理的关系。方法采用链霉菌素-生物素(SP)免疫组织化学法及免疫印记反应(Western blot)对45例肝细胞癌组织、39例癌旁组织及37例非肿瘤肝组织标本进行检测分析。结果 p14ARF蛋白在肝癌肿瘤组织中表达缺失率为84．4％(38／45),p14ARF蛋白I级-Ⅱ级(高分化)病例p14ARF蛋白缺失率为 44．4％,Ⅲ级-Ⅳ级(低分化)病例p14ARF蛋白缺失率为94．4％(P<0．05)。结论 p14ARF蛋白表达缺失与肝细胞癌的发生、发展有一定相关性。提示 p14ARF基因的失活可能是肝细胞癌恶性演变的重要因素。
Objective To investigate the loss of p14ARF expression in human hepatocellular carcinoma and its relationship with clinicopathological features. Methods 45 cases of hepatocellular carcinoma, 39 cases of paracancerous tissues and 37 cases of non-tumor liver tissues were detected by streptavidin-biotin (SP) immunohistochemistry and Western blot. Results The deletion rate of p14ARF protein in hepatocellular carcinoma was 84.4% (38/45). The deletion rate of p14ARF protein in grade Ⅰ-Ⅱ (well-differentiated) p14ARF protein was 44.4%, grade Ⅲ-Ⅳ Poorly differentiated) cases of p14ARF protein deletion rate was 94.4% (P <0.05). Conclusion The lack of p14ARF protein expression is associated with the occurrence and development of hepatocellular carcinoma. Tip p14ARF gene inactivation may be an important factor in the evolution of malignant hepatocellular carcinoma.