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目的比较子宫颈鳞癌及腺癌细胞对氧化应激的抵抗并探讨其机制。方法①H2O2处理子宫颈鳞癌(Siha、SW756)及腺癌(Hela、GH329)细胞,噻唑蓝(MTT)法检测两型细胞的增殖水平;②用250、250、650及800μmol/L H2O2分别处理子宫颈鳞癌(Siha、SW756)及腺癌(Hela、GH329)细胞,流式细胞仪检测细胞内活性氧(ROS)水平,酶标仪检测细胞内还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)水平,分光光度计检测细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GPx)活性。结果在不进行处理的情况下,子宫颈鳞癌(Siha、SW756)细胞较子宫颈腺癌(Hela、GH329)细胞有较低的ROS水平、GSH含量及GSH/GSSG比值,较高的GSSG水平及SOD和GPx活性。在氧化应激条件下,子宫颈鳞癌(Siha、SW756)细胞的GSH水平及GSH/GSSG比值明显下降,SOD、CAT和GPx活性升高;子宫颈腺癌(Hela、GH329)细胞的GSH水平及GSH/GSSG比值略有下降,SOD、CAT和GPx活性升高。结论子宫颈鳞癌及腺癌细胞处于不同的氧化还原状态,子宫颈鳞癌细胞较子宫颈腺癌细胞抗氧化能力弱,这可能与H2O2处理后鳞癌细胞ROS较腺癌细胞增多明显及两型细胞抗氧化系统有不同的应答有关。
Objective To compare the resistance to oxidative stress in squamous cell carcinoma and adenocarcinoma of the cervix and to explore its mechanism. Methods ①H2O2 treatment of cervical cancer cells (Siha, SW756) and adenocarcinoma (Hela, GH329) cells, MTT method to detect the proliferation of two types of cells; ② 250,250,650 and 800μmol / L H2O2 were treated (Siha, SW756) and adenocarcinoma (Hela, GH329) were detected by flow cytometry. The levels of reactive oxygen species (ROS) in cells were detected by flow cytometry. Glutathione Glutathione (GSSG), and the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in cells were detected by spectrophotometer. Results Compared with Hela, GH329 cells, there was a lower ROS level, GSH content and GSH / GSSG ratio, higher GSSG level in untreated cervical cancer cells (Siha, SW756) And SOD and GPx activity. Under oxidative stress, GSH level and GSH / GSSG ratio of Siha (SW756) cells were significantly decreased, while activities of SOD, CAT and GPx were increased. GSH levels in cells of cervical adenocarcinoma (Hela, GH329) And GSH / GSSG ratio decreased slightly, SOD, CAT and GPx activity increased. Conclusions Cervical squamous cell carcinoma and adenocarcinoma cells are in different redox states, and cervical squamous carcinoma cells have weaker anti-oxidative ability than cervical adenocarcinoma cells. This may be due to the obvious increase of ROS in squamous cell carcinoma compared with that in adenocarcinoma treated with H2O2 and two Cellular antioxidant systems have different responses related.