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随着淡水资源的匮乏,干旱已成为影响玉米产量众多因素中最主要的非生物因素。而植物的耐旱性又属于数量遗传性状,这给广大遗传育种工作者带来了一个很大的难题。SKIP基因属于RNA调控基因,它参与调节生物体内多种RNA的剪切调控,进一步调整植物状态,应对逆境环境。利用BLAST搜索玉米基因组,得到相似度99%的玉米基因,命名为ZmSKIP。对该基因克隆和生物信息学分析,构建过量表达载体,使用原位转化法得到过表达该基因的转基因株。对转基因株进行耐旱性实验,并使用ELISA方法检测转基因株的ABA含量。结果显示,转基因株中ABA相对野生型上升明显,具有更高的耐旱性。初步推断ZmSKIP在玉米中可能调控相应的耐旱基因表达从而调节植物对逆境的适应能力。
With the lack of freshwater resources, drought has become the most important abiotic factor that affects many factors of maize production. The drought tolerance of plants belongs to the quantitative genetic traits, which brings a big problem to the genetics and breeding workers. SKIP gene belongs to RNA regulation gene, which is involved in the regulation of cutting and regulation of various RNAs in the organism to further adjust the state of plants and deal with adverse environment. Using the BLAST search of the maize genome, a corn gene of 99% similarity was obtained and named ZmSKIP. The gene cloning and bioinformatics analysis, construction of overexpression vector, the use of in situ transformation obtained over-expression of the gene transgenic strains. Drought tolerance experiments were conducted on the transgenic lines and the ABA content of the transgenic lines was measured by ELISA. The results showed that compared with the wild-type ABA transgenic lines increased significantly, with higher drought tolerance. It is preliminarily concluded that ZmSKIP may regulate the expression of drought tolerant genes in maize so as to regulate plant adaptability to adverse conditions.