血液与女性生殖道中HIV-1RNA和DNA的纵向及横向研究

• 作 者: Iversen A.K.N. Attermann J.stoft J. 侯巍
• 机 构: Human Immunology Unit, Weatherall Inst. of Molec. Medicine, John Radcliffe Hospital, OX3 9AD, Oxford
• 出 处: 世界核心医学期刊文摘(妇产科学分册)
• 发表日期: 2005年04期
• 关键词: 横向研究 生殖道 纵向 生殖道分泌物 血液 RNA 宫颈 女性个体 变量 β趋化因子

Objective: To examine if correlates of HIV-1 genital shedding in crosssectional studies can be used to determine the risk of shedding in individual HIV-1-positive women. Study design: Longitudinal samples from blood and cervix were obtained from 18 HIV-1 infected women, and HIV-1 RNA and cellassociated DNA virus, and betachemokine levels, were measured. Associations between variables were analyzed at both individual and group level. Results: The variation over time was 2.9-, 2.1-, and 2.3-fold in plasma RNA, PBMC DNA and cervical RNA load, respectively, and reached 6.2-fold in cervical DNA load. Differences were observed between associations in individualand grouplevel comparisons, suggesting that a separate reservoir of HIV replication may exist in the genital tract of some women, which is influenced by local environmental factors. Conclusions: Our study underscores the importance of caution during contact with genital fluids at all stages of infection and disease regardless of treatment and HIV-1 blood loads.

Objective: To examine if correlates of HIV-1 genital shedding in crosssectional studies can be used to determine the risk of shedding in individual HIV-1-positive women. Study design: Longitudinal samples from blood and cervix were obtained from 18 HIV- 1 infected women, and HIV-1 RNA and cellassociated DNA virus, and betachemokine levels, were measured. Associations between variables were analyzed at both individual and group levels. Results: The variation over time was 2.9-, 2.1 -, and 2.3-fold in plasma RNA, PBMC DNA and cervical RNA load, respectively, and reached 6.2-fold in cervical DNA load. Differences were observed between associations in individualand grouplevel comparisons, suggesting that a separate reservoir of HIV replication may exist in the genital tract of some women, which is influenced by local environmental factors. Conclusions: Our study underscores the importance of caution during contact with genital fluids at all stages of infection and disease re gardless of treatment and HIV-1 blood loads.