腺病毒和腺伴随病毒基因元件提高白细胞介素-6在真核细胞中的表达效率

• 作 者: 刘红涛 袁勇 张颖妹 狄春辉 王应 马大龙
• 机 构: 北京医科大学免疫学系
• 出 处: 北京医科大学学报
• 发表日期: 1998年06期
• 关键词: 白细胞介素6/代谢 遗传载体 腺病毒 人 克隆 分子

目的：构建高效真核表达载体以提高细胞因子在真核细胞中的表达效率。方法：利用基因克隆技术构建了两个新的白细胞介素６（ＩＬ６）真核表达载体ｐＡｄＣＩＩＬ６和ｐ３３５ＣＩＬ６。将它们瞬时转染ＣＯＳ７细胞后，利用ＩＬ６依赖株７ＴＤ１对上清中的ＩＬ６进行检测。结果：两种新建载体分别能提高ＩＬ６在真核细胞中的表达效率５．５和３．４倍。结论：腺病毒的病毒相关Ⅰ和ⅡＲＮＡ（ｖｉｒｕｓａｓｏｃｉａｔｅｄⅠａｎｄⅡＲＮＡ，ＶＡⅠａｎｄＶＡⅡ）和腺伴随病毒（ａｄｅｎｏａｓｏｃｉａｔｅｄｖｉｒｕｓ，ＡＡＶ）的倒转末端重复序列（ｉｎｖｅｒｔｅｄｔｅｒｍｉｎａｌｒｅｐｅａｔ，ＩＴＲ）能提高细胞因子在真核细胞中的表达

Objective: To construct efficient eukaryotic expression vector to improve the expression of cytokines in eukaryotic cells. Methods: Two new interleukin6 (IL6) eukaryotic expression plasmids pAdCIIL6 and p335CIL6 were constructed by gene cloning technique. After they were transiently transfected COS 7 cells, the use of IL 6 dependent strains 7TD1 supernatant IL  6 were detected. Results: The two new vectors could improve the expression efficiency of IL6 in eukaryotic cells by 5.5 and 3.4 times, respectively. CONCLUSION: The inverted terminal resequence (ITR) of adenovirus-associated viral vaso-associated I and II (VAⅠandVAⅡ) and adeno-associated virus (AAV) can increase the expression of cytokines in eukaryotic cells