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目的通过抑制NF-κB信号通路,探讨其对原代培养小胶质细胞TREM2表达的影响。方法取新生C57BL/6小鼠大脑皮质和海马,进行原代培养,将培养至10 d的混合胶质细胞分离,纯化出小胶质细胞,将其分为空白组和1、2、4、6、8、10μmol/L NF-κB抑制剂(PDTC)组。继续培养24 h后,CCK8法检测小胶质细胞存活率筛选适宜PDTC浓度,Western blotting法检测小胶质细胞TREM2的表达。结果与空白组相比,给予NF-κB抑制剂后,原代培养的小胶质细胞表达TREM2的量显著增加(P<0.01)。结论抑制NF-κB信号通路增加小胶质细胞TREM2的表达,进而可能有助于中枢神经系统中炎症的调控。
Objective To investigate the effects of NF-κB signaling pathway on TREM2 expression in primary cultured microglia cells. Methods The primary cultured C57BL / 6 mice were cultured in primary cortex and hippocampus. The mixed glial cells cultured for 10 days were separated and purified. The microglia were divided into blank group and 1, 2, 4, 6, 8, 10μmol / L NF-κB inhibitor (PDTC) group. After cultured for 24 h, the appropriate concentration of PDTC was detected by CCK8 assay and microglia TREM2 expression was detected by Western blotting. Results Compared with the blank group, the amount of TREM2 in primary cultured microglia significantly increased (P <0.01) after NF-κB inhibitor administration. Conclusion Inhibition of NF-κB signaling pathway increases the expression of TREM2 in microglia cells, which in turn may contribute to the regulation of inflammation in the central nervous system.