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将非放射性标记物地高辛甙元用随机引物法由ｐＢＲ３２２－２ＨＢＶ／ＥｃｏＲⅠ酶切片段（３．２ｋｂ）制备ＨＢＶ－ＤＮＡ探针及由ＨＢＶ／ＢａｍＨⅠ、ＢｇｌⅡ酶切片段（０．５９ｋｂ）制备ＨＢｘ－ＤＮＡ探针。将Ｄｉｇ－ＨＢＶＤＮＡ探针用斑点杂交、Ｓｏｕｔｈｅｒｎ转膜杂交检测筛选肝细胞性肝癌ＨＢＶ－ＤＮＡ整合型标本，进而用Ｄｉｇ－ＨＢｘＤＮＡ探针检测ＨＢＶ－ＤＮＡ纯整合型肝细胞性肝癌中ＨＢｘ的存在情况。结果显示，７５％（３３／４４）的肝癌ＤＮＡ中有ＨＢＶ－ＤＮＡ存在，其中，以纯整合型方式存在的为６３．６％（２１／３３），混合型为３６．４％（１２／３３），无游离型。纯整合型ＨＢＶ－ＤＮＡ片段中，ＨＢｘ－ＤＮＡ阳性率为９０．５％（１９／２１）。提示ＨＢＶ在肝细胞性肝癌的发生中起着重要的作用，ＨＢＶ－ＤＮＡ可能以片段性整合的方式存在于肝癌细胞染色体上，其中以ＨＢｘ基因的整合为主。
The non-radioactive marker digoxigenin was prepared from the pBR322-2 HBV/EcoRI digested fragment (3.2 kb) using the random primer method and the HBV-DNA probe was prepared from the HBV/BamHI and BglII fragment (0.59 kb). DNA probes. The Dig-HBV DNA probe was used to detect HBV-DNA integrated specimens of hepatocellular carcinoma by dot blot hybridization and Southern blot hybridization, and then the presence of HBx in HBV-DNA pure integrated hepatocellular carcinoma was detected by Dig-HBx DNA probes. . The results showed that HBV-DNA was present in 75% (33/44) of the liver cancer DNA, of which 63.6% (21/33) existed in a purely integrated manner and 36.4% (12/33) in a mixed type. ), no free type. In the purely integrated HBV-DNA fragment, the positive rate of HBx-DNA was 90.5% (19/21). It is suggested that HBV plays an important role in the development of hepatocellular carcinoma, and HBV-DNA may exist in the chromosome of hepatoma cells in a fragment-integrated manner. Among them, the integration of HBx genes is predominant.