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导数分光光度法与普通分光光度法不同,它不是测试吸光度(A),而是测试吸光度对波长的导数输出信息(d~nA/dλ~n),因而它具有许多有用的特点:有分辨重叠峰及辨认、放大“肩峰”的本领;能准确确定宽谱吸收带的极大位置;消除背景干扰;大幅度提高灵敏度以及进行定量测定等。引入导数技术后使分光光度法无论从提高灵敏度、选择性、准确度或是实现多元素同时测定等方面都可能有所突破,将成为一种很有前途的分析方法而被广泛应用于各领域。
Unlike spectrophotometry, derivative spectrophotometry does not test absorbance (A) but rather absorbs the derivative of wavelength (d ~ nA / dλ ~ n) so it has many useful properties: Peak and identify, enlarge “Shoulder” ability; Can accurately determine the location of the broad band absorption; Eliminate background interference; A substantial increase in sensitivity and quantitative determination. The introduction of derivative technology makes spectrophotometry breakthrough in terms of improving sensitivity, selectivity, accuracy or simultaneous determination of multiple elements and will be a promising analytical method widely used in various fields .