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应用兔抗微丝蚴抗体及抗班氏丝虫微丝蚴代谢抗原(ES34株)或抗马来丝虫3期幼虫抗原(HC11株)单克隆抗体(McAb)酶联免疫吸附试验(ELISA)双抗体法检测人体丝虫病循环抗原时,微丝蚴血症者阳性率分别为94.5％(103/109)及89.0％(97/109),且ES34株单克隆抗体ELISA检测结果显示微丝蚴密度与抗原滴度呈正相关;部分微丝蚴血症者尿液中亦可测得丝虫抗原;晚期丝虫病人阳性率分别为57.4％(31/54)及61.1％(33/54);获自美国及中国贵阳非丝虫病流行区正常人血清阳性率分别为0～4.1％及2.8～4.1％;30份肠道蠕虫感染者血清全部为阴性。血清中丝虫循环抗原的存在似与活动性感染有关。
Rabbit anti-Filaria antibodies and anti-filamentous microfilariae metacercariae antigens (ES34 strain) or anti-Malayi 3 larvae antigen (HC11 strain) monoclonal antibody (McAb) enzyme-linked immunosorbent assay (ELISA) The positive rate of microfilaremia was 94.5% (103/109) and 89.0% (97/109) respectively when using double antibody method to detect human filariasis circulating antigen, and the results of ELISA of ES34 monoclonal antibody showed that microfilament The density of larvae was positively correlated with the antigen titers. Some microfilaremia patients could also detect filarial antigen in urine. The positive rates of late filariasis were 57.4% (31/54) and 61.1% (33/54), respectively The seroprevalence of normal people from non-filariasis epidemic areas in the United States and China was 0-4.1% and 2.8-4.1%, respectively. All the serums from 30 gut worms were negative. The presence of circulating antigens of filarial worms in serum seems to be associated with active infection.