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目的 观察不同损毁程度帕金森病 (PD)小鼠模型的纹状体多巴胺转运蛋白 (DAT)功能变化 ,探讨12 5I 甲基 3β (4 碘苯基 )托烷 2 β 羧酸盐 (β CIT)DAT显像的价值。 方法 根据注射MPTP天数不同 ,分为 1,3,5和 7d模型组和对照组 ,静脉注射12 5I β CIT 148kBq 2h后行放射自显影。高效液相色谱 电化学法 (HPLC ECD)检测纹状体多巴胺 (DA)及其代谢产物含量。免疫组化酪氨酸羟化酶 (TH)染色观察黑质和纹状体的病理变化。结果 与对照组相比 ,MPTP损毁的各组PD小鼠模型的纹状体 /皮层放射性比值分别降低 2 0 % ,42 % ,45 %和 5 2 % ;纹状体DA含量分别降低 47% ,75 % ,95 %和 95 % ;免疫组化TH染色可见黑质TH阳性神经元数量随MPTP损毁程度的加重而进一步减少。结论 不同损毁程度的PD小鼠模型DAT功能变化与生化和病理改变相一致 ,β CITDAT功能显像有助于PD的早期诊断和病情监测
Objective To investigate the changes of dopamine transporter (DAT) function in striatum of Parkinson’s disease (PD) mice with different degrees of damage, and to investigate the effects of 125I methyl 3β (4-iodophenyl) tropane 2β carboxylate (β CIT) The value of DAT imaging. Methods According to the different days of MPTP injection, the rats were divided into 1, 3, 5 and 7 days model group and control group. After intravenous injection of 125I β CIT 148kBq for 2 hours, autoradiography was performed. The contents of dopamine (DA) and its metabolites in striatum were determined by HPLC-ECD. Immunohistochemical tyrosine hydroxylase (TH) staining was used to observe the pathological changes of substantia nigra and striatum. Results Compared with the control group, the striatal / cortical radioactivity ratios of PD mice damaged by MPTP decreased by 20%, 42%, 45% and 52%, respectively. The striatal DA content decreased by 47% 75%, 95% and 95%, respectively. Immunostaining for TH staining showed that the number of TH positive substantia nigra neurons decreased further with the severity of MPTP damage. Conclusions The changes of DAT function in PD mice with different degrees of damage are consistent with the biochemical and pathological changes. Functional imaging of β CITDAT is helpful for the early diagnosis and monitoring of PD