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以20个辽宁省主栽香菇菌株为试材,采用ISSR分子标记方法,研究了20个菌株的遗传差异性,并采用NTSYS-PCR软件进行聚类分析。结果表明:从30个随机引物中筛选出9个ISSR备用引物,并用这9个引物对供试香菇菌株基因组DNA进行了ISSR-PCR扩增,共扩增出72条清晰条带,其中多态性条带占83.3%。通过聚类分析发现,异化系数在0.36为阈值时,20个菌株聚为4个组群。
Twenty main strains of Lentinula edodes in Liaoning Province were used as test materials, genetic diversity of 20 strains was studied by ISSR molecular marker method, and clustering analysis was carried out by NTSYS-PCR software. The results showed that 9 ISSR spare primers were screened from 30 random primers, and the 9 primers were used to amplify the genomic DNA of the tested shiitake mushrooms by ISSR-PCR. A total of 72 clear bands were amplified, of which polymorphic bands Sexual bands accounted for 83.3%. Cluster analysis showed that when the alienation coefficient was 0.36, the 20 strains clustered into four groups.