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目的观察3-乙酰基-4-乙酰氧基苯并噁唑-2-酮(3-acetyl-4-acetoxy-2-benzoxazolone,TC-3)对四氯化碳所致小鼠急性肝损伤的保护作用,并探讨其疗效机制。方法采用腹腔注射体积分数0.15%四氯化碳制备小鼠急性肝损伤模型,3-乙酰基-4-乙酰氧基苯并噁唑-2-酮各剂量组灌胃给药,检测不同剂量的3-乙酰基-4-乙酰氧基苯并噁唑-2-酮对肝损伤小鼠血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶活性以及肝组织匀浆中超氧化物歧化酶活性、丙二醛和还原型谷胱甘肽含量的影响,同时光镜下观察肝组织的病理变化。结果 3-乙酰基-4-乙酰氧基苯并噁唑-2-酮能明显降低四氯化碳致急性肝损伤小鼠血清血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶的活性,降低肝匀浆丙二醛的含量,同时提高肝组织中超氧化物歧化酶、谷胱甘肽的活性,并能减轻四氯化碳对肝组织的病理损伤。结论 3-乙酰基-4-乙酰氧基苯并噁唑-2-酮对四氯化碳致小鼠急性肝损伤具有一定的保护作用,其保护机制可能与清除自由基、抗脂质过氧化作用有关。
Objective To investigate the effect of 3-acetyl-4-acetoxy-2-benzoxazolone (TC-3) on carbon tetrachloride-induced acute liver injury in mice Protective effect, and explore its mechanism of efficacy. Methods The mouse model of acute liver injury was induced by intraperitoneal injection of carbon tetrachloride (0.15%), and each dose of 3-acetyl-4-acetoxybenzoxazol-2-one Effect of 3-acetyl-4-acetoxybenzoxazol-2-one on serum alanine aminotransferase, aspartate aminotransferase activity and superoxide dismutase activity in liver homogenate of mice with hepatic injury , Malondialdehyde (MDA) and reduced glutathione (GSH), and observed the pathological changes of liver tissue under light microscope. Results 3-Acetyl-4-acetoxybenzoxazol-2-one can significantly reduce the activity of serum alanine aminotransferase and aspartate aminotransferase in mice with acute hepatic injury induced by carbon tetrachloride , Reduce the content of malondialdehyde in liver homogenate, increase the activity of superoxide dismutase and glutathione in liver tissue at the same time, and reduce the pathological damage of liver tissue by carbon tetrachloride. Conclusion 3-Acetyl-4-acetoxybenzoxazol-2-one can protect mice from acute liver injury induced by carbon tetrachloride. The protective mechanism may be related to scavenging free radical, anti-lipid peroxidation Relevant role.