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目的:建立测定余甘子喉片中没食子酸含量的HPLC方法。方法:色谱柱:Agilent ZORBAX Eclipse XDB-C_(18)(150mm×4.6mm,5μm)柱;流动相:甲醇-水-磷酸(5:95:0.05);流速:1.0ml·min~(-1);检测波长:280nm;柱温:30℃,外标定量法。结果:没食子酸在0.09～0.56 mg·ml~(-1)呈良好的线性关系(r=0.999 9),平均回收率为99.8%(n=6),RSD=0.27%。结论:本法操作简单,灵敏,准确,可以用于余甘子喉片的质量控制。
Objective: To establish an HPLC method for determination of gallic acid content in Phyllanthus emblica. Method: Column: Agilent ZORBAX Eclipse XDB-C_(18) (150mm×4.6mm, 5μm) column; mobile phase: methanol-water-phosphoric acid (5:95:0.05); flow rate: 1.0ml·min~(-1) Detection wavelength: 280 nm; Column temperature: 30 °C, external standard quantitative method. RESULTS: The linearity of gallic acid was between 0.09 and 0.56 mg·ml -1 (r=0.999 9). The average recovery was 99.8% (n=6) and RSD was 0.27%. Conclusion: This method is simple, sensitive, accurate and can be used for quality control of Phyllanthus emblica lozenges.