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应用[α—~(32)P]—HPV16 DNA探针和Southern blo核酸杂交技术检测了154份宫颈活检组织标本。在低限制性条件和高限制性条件下;宫颈癌组织HPV16 DNA杂交的阳性率分别为50.86％(59/116)和37.07％(43/116),且鳞癌与腺癌之间无显著性差异(P>0.90);正常宫颈组织的阳性率则分别为5.56％(2/36)和0.00%(0/36)。宫颈癌组织HPV16 DNA杂交的阳性率均显著地高于正常宫颈组织(P<0.001)。另外,在2例宫颈间变组织中检出1例HPV16 DNA阳性杂交结果。上述给果提示:HPV16及其相关型感染与宫颈癌之间可能存在病因学联系。
A total of 154 cervical biopsy specimens were detected by [α- ~ (32) P] -HPV16 DNA probe and Southern blot hybridization. The positive rates of HPV16 DNA hybridization in cervical cancer tissues were 50.86% (59/116) and 37.07% (43/116) respectively, with no significant difference between squamous cell carcinoma and adenocarcinoma under low and high restrictive conditions (P> 0.90). The positive rates of normal cervical tissue were 5.56% (2/36) and 0.00% (0/36) respectively. The positive rates of HPV16 DNA hybridization in cervical cancer tissues were significantly higher than those in normal cervical tissues (P <0.001). In addition, one case of HPV16 DNA positive hybridization was detected in 2 cases of cervical intervertebral body. The above results suggest that there may be etiological relationship between HPV16 and its associated infection and cervical cancer.