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Objective:To investigate the antiplasmodial activity of Phyllanthus niniri(P.niruri) methanol extract(ME) and its fractions in mice.Methods:P.niruri methanol extract and its chloroform. ethanol and aqueous portions were tested against chloroquine-sensitive Plasmodium berghei berghei in early,established and repository models of infection using Knight and Peter’s 4-day suppressive model,Rvlev and Peters curative model and Peters prophylactic model respectively. Results:Chemosuppression of parasitemia(37.65%-50.53%) was elicited by 100-400 mg/kg(b.w.) of ME.Al doses of 100 mg/kg b.w..the chloroform fraction(F1) significantly(P<0.01) suppressed parasitaemia by 85.29%,while ethanol and aqueous fractions(F2 and F3.respectively) elicited 67.06%and 51.18%chemosuppression.the most active fraction.F1 was selected for further antiplasmodial screening.In established infection.ME reduced parasitaemia(15.81%- 62.96%) while F1 significantly(P<0.01) reduced parasitaemia(44.369—90.48%).with effects comparable to that of chloroquine(96.48%).The prophylactic antiplasmodial activity of ME(92.50%suppression) was also significant(P<0.01) and was more effective than pyrimethamine(85.00%).additionally, cell membrane integrity of non-parasitized erythrocytes intubated with 125-500 mg/mL F1 was maintained.Conclusions:These findings indicate the antiplasmodial efficacy of P.niruri methanol extract,and the localization of this effect in its chloroform fraction.
Objective: To investigate the antiplasmodial activity of P. niruri methanol extract (ME) and its fractions in mice. Methods: P. niruri methanol extract and its chloroform. Ethanol and aqueous portions were tested against chloroquine-sensitive Plasmodium berghei berghei in early, established and repository models of infection using Knight and Peter’s 4-day suppressive model, Rvlev and Peters curative model and Peters prophylactic model respectively. Results: Chemosuppression of parasitemia (37.65% -50.53%) was elicited by 100-400 mg / The chloroform fraction (F1) significantly (P <0.01) suppressed parasitaemia by 85.29%, while ethanol and aqueous fractions (F2 and F3.respectively) elicited 67.06% and 51.18% reduced to parasitaemia (44.369-90.48%) with F1 significantly (14.369-90.48%) while F1 was was selected for further antiplasmodial screening. Established reduced effects of parasitaemia (15.81% - 62.96%Furthermore, cell membrane integrity of non-parasitized erythrocytes (96.48%). The prophylactic antiplasmodial activity of ME (92.50% suppression) was also significantly (P <0.01) and was more effective than pyrimethamine intubated with 125-500 mg / mL F1 was kept. Conclusions: These findings indicate that the antiplasmodial efficacy of P. niruri methanol extract, and the localization of this effect in its chloroform fractions.