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在运用回交法进行非AA基因组野生稻有利基因的转移与利用时,最大的困难就是极难得到足量的BC1F1植株。用柱头外露率高的保持系金23B作母本,小粒野生稻作父本进行杂交,经胚拯救得到了杂种F1植株;再用杂种F1植株作母本,相应的保持系作父本,采用类似于杂交稻制种的田间种植方法进行回交,获得了大量的远缘杂交回交一代的幼胚,经胚拯救得到了134株BC1F1植株。用80对SSR标记对双亲、F1和BC1F1植株进行了鉴定,表明杂交和回交获得的都是真杂种,而且F1和BC1F1植株SSR标记的带型大部分一致。农艺性状鉴定表明,BC1F1植株的许多形态性状与杂种F1相似,但是在生长习性、株高、叶长、穗长和每穗颖花数等方面差异明显。
The greatest difficulty in using the backcross method to transfer and utilize favorable genes for non-AA genome wild rice is that it is extremely difficult to obtain sufficient quantities of BC1F1 plants. Using the maintainer line 23B with high stigma exsertion rate as the male parent and the mini-wild rice as the male parent, the hybrid F1 plants were obtained through embryo rescue. The hybrid F1 plants were used as the female parent and the corresponding maintainer line was used as the male parent. Similar to the field planting method of hybrid rice seed production backcross, a large number of distant hybridization backcross generation of immature embryos obtained by embryo rescue 134 BC1F1 plants. Eighty pairs of SSR markers were used to identify the parents, F1 and BC1F1 plants, which indicated that both hybrids and backcross were true hybrids, and most of the SSR markers in F1 and BC1F1 plants belonged to the same genotype. Identification of agronomic traits showed that many morphological traits of BC1F1 plants were similar to hybrid F1, but significant differences were observed in growth habit, plant height, leaf length, spike length and spikelet number per spike.