STUDIES ON THE PROPERTY OF SULFHYDRYL BINDING SITE ON THE LUNG NORMAL AND CANCER CELL MEMBRANE OF CH

• 作 者: 忻文娟 赵保路 张建中
• 机 构: Institute of Biophysics, Academia Sinica, Beijingrrrrrrrrn,Institute of Biophysics, Academi
• 出 处: Science in China,Ser.B
• 发表日期: 1984年10期
• 关键词: CELL SPIN label weakly rotational bottle aired finer split dissolved

In this paper, five maleimide spin labels with different chain lengths were used to studythe properties of binding sites of sulfhydryl groups on the meanbrane proteins of normal cellV_79 and cancer cells V_79-B_1 in the lung of Chinese hamster. The ratio of the strongly immobi-lized component to the weakly immobilized eomponent (s/w) and the rotational correlation time(τ_e) were calculated on the basis of ESR spectra. From the varying of the s/w and τ_c withthe ehain lengths of spilt labels it was deducted that the binding sites of sulfhydrylgroups on V_79 and V_79-B_1 membrane proteins were of eonical shape and that the binding sitesof sulfhydryl groups on V_79 membrane protein were deeper and narrower than those on V_79-B_1.

In this paper, five maleimide spin labels with different chain lengths were used to study the properties of binding sites of sulfhydryl groups on the meanbrane proteins of normal cell V_79 and cancer cells V_79-B_1 in the lung of Chinese hamster. The ratio of the strongly immobi- lized component to the weakly immobilized eomponent (s / w) and the rotational correlation time (τ_e) were calculated on the basis of ESR spectra. From the varying of the s / w and τ_c with the ehain lengths of spilled labels it was deducted that the binding sites of sulfhydryl groups on V_79 and V_79-B_1 membrane proteins were of eonical shape and that the binding sites of sulfhydryl groups on V_79 membrane protein were deeper and narrower than those on V_79-B_1.