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目的研究耐药性癫患者的载脂蛋白E基因多态性,并探究影响耐药性癫形成机制的遗传因素。方法取耐药性癫患者肘静脉血,用PCR-RFLP技术检测ApoE基因多态性,并与非耐药性癫和正常对照组进行比较分析。结果耐药性癫组中ε4等位基因的频率为(16.9)),明显高于非耐药性癫组(8.2)和正常对照组,其差异有统计学意义(P<0.05),而ε2,ε3等位基因的频率三组之间差异无统计学意义(P>0.05);病例组与正常对照组ApoE基因多态性有显著性差异(P<0.01)。结论ApoEε4等位基因可增加耐药性癫发生的危险性。
Objective To study the polymorphism of apolipoprotein E gene in patients with drug-resistant epilepsy and explore the genetic factors that affect the mechanism of drug-resistant epilepsy. Methods The elbow vein blood was collected from patients with drug-resistant epilepsy. The polymorphism of ApoE gene was detected by PCR-RFLP and compared with non-drug resistant epilepsy and normal controls. Results The frequency of ε4 alleles in drug-resistant epilepsy group was (16.9), which was significantly higher than that in non-drug-resistant epilepsy group (8.2) and normal control group (P <0.05) There was no significant difference in the frequencies of ε2 and ε3 alleles between the three groups (P> 0.05). The ApoE gene polymorphisms in the cases and normal controls were significantly different (P <0.01). Conclusion ApoE ε 4 alleles may increase the risk of drug-resistant epilepsy.