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为明确江苏地区Q型烟粉虱的遗传多样性及其入侵来源,基于mt DNA COI基因序列,对2010、2011年采自江苏13个市的Q型烟粉虱种群进行了单倍型分析。结果显示,江苏地区Q型烟粉虱有4个单倍型,分别为单倍型Q1、Q2、Q3、Q4,不同单倍型的分布和发生频率不同,其中单倍型Q2是13个地理种群的共享单倍型,2010、2011年的发生频率均超过50%;单倍型Q1和Q3分别是部分地理种群的共享单倍型,发生频率较低;单倍型Q4仅在扬州种群中出现,发生频率最低;单倍型Q1和Q3亲缘关系较近,单倍型Q2和Q4亲缘关系较近,表明各地理种群间既有一定的基因交流,也存在一定程度的遗传分化;系统发育分析表明,江苏地区的Q型烟粉虱可能来源于西部地中海地区,与日本的Q型烟粉虱具有相同的入侵来源。
To determine the genetic diversity of Q-biotype Bemisia tabaci (Gennadius) and their sources of invasion in Jiangsu Province, based on the mt DNA COI gene sequences, haplotype analysis was conducted on Q-biotype Bemisia tabaci (Gennadius) population collected from 13 cities in Jiangsu Province in 2010 and 2011. The results showed that there were 4 haplotypes of Q-biotype Bemisia tabaci in Jiangsu Province, which were haplotypes Q1, Q2, Q3 and Q4, respectively. The distribution and frequency of different haplotypes were different. Among them, haplotype Q2 was 13 geographies The haplotypes shared more than 50% in 2010 and 2011. The haplotypes Q1 and Q3 were shared haplotypes of partial geographic populations respectively, with a lower frequency of occurrence. The haplotype Q4 was only found in Yangzhou population Which occurred at the lowest frequency. The haplogroups Ql and Q3 had a close genetic relationship, and the haplogroups Qz and Q4 had a close genetic relationship, indicating that there was some gene exchange and some genetic differentiation between the geographic populations. The analysis showed that Q-biotype Bemisia tabaci in Jiangsu Province may originated from the western Mediterranean region and had the same source of invasion as Q-type B. tabaci in Japan.