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叶下珠(phy)属于大戟科油柑属植物。国外曾报道该物有护肝作用。并能使HBsAg阴转。我们用原代培养的大鼠肝细胞CCl_4损伤模型,研究重庆产phy对肝细胞的保护作用及作用机制。 一、肝细胞的分离及培养:胶原酶灌流法分离大鼠肝细胞,置CO_2培养箱培养4小时,换液后加CCl_4(终浓度10mmol/L)给药组在肝细胞与药物作用1h后,再加入与损伤组等量CCl_4。
Phyllostachys (Phyllostachys pubescens) belongs to genus Euphorbiaceae. Foreign reports have been the role of liver protection. And can make HBsAg negative. We use the primary cultured rat hepatocyte CCl 4 injury model to study the protection of phytopathogenic hepatocytes in Chongqing and its mechanism of action. First, the isolation and culture of hepatocytes: Rat liver cells were isolated by collagenase perfusion method and cultured for 4 hours in CO 2 incubator. After treated with CCl 4 (final concentration 10 mmol / L) for 1 hour after hepatocyte and drug treatment , And then added with the same amount of injury group CCl_4.