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利用高能粒子处理紫花苜蓿干种子,观测龙牧803苜蓿M2代在秋末冬初低温条件下叶片游离脯氨酸含量(Pro)、过氧化物歧化酶活性(SOD)、可溶性糖含量(LSSC)、叶绿素a含量(Chla)和叶绿素b含量(Chlb)。结果表明,高能粒子处理在秋末冬初低温条件下M2代的抗寒性指标显著高于对照(P<0.05);随着辐照剂量的增大,M2代叶片Pro含量、SOD酶活性、LSSC含量、Chl含量明显递减。低剂量辐照下M2代获得的抗寒性强于高剂量辐照,由此确定苜蓿的最佳高能粒子辐照剂量为145 Gy,其次是195 Gy。
The dry seeds of alfalfa were treated with high energy particles and the contents of free proline (Proline), superoxide dismutase (SOD), soluble sugar content (LSSC) Chlorophyll a content (Chla) and chlorophyll b content (Chlb). The results showed that the cold resistance index of M2 generation was significantly higher than that of the control (P <0.05) at low temperature in early autumn and early winter in high energy particle treatment. Progenin content, SOD activity, LSSC Content, Chl content decreased significantly. The cold resistance of M2 generation under low-dose irradiation was stronger than that of high-dose irradiation, so the optimal dose of high-energy particle irradiation of alfalfa was 145 Gy, followed by 195 Gy.