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目的 :研究抑癌基因 p1 6在胰腺癌中的表达及其意义。方法 :采用免疫组化 LSAB法 ,以p1 6多克隆抗体检测胰腺腺癌 2 8例 ,正常胰腺组织 6例中 P1 6蛋白的表达。结果 :1正常胰腺组织中 P1 6蛋白的表达率与胰腺腺癌中 P1 6蛋白的表达率比较差异显著 (P<0 .0 5)。 2胰腺腺癌中P1 6蛋白的表达率与病人的年龄、性别、肿瘤大小等因素无相关性 (P>0 .0 5)。 3不同分化程度的胰腺腺癌中 P1 6蛋白的表达率差异显著 (P<0 .0 5)。 4胰腺腺癌中 P1 6蛋白的表达率在是否有淋巴结转移两组间比较无统计学意义 (P>0 .0 5)。 5胰腺腺癌中 P1 6蛋白的表达率与病人术后生存期长短之间比较呈正相关 (P<0 .0 5)。结论 :抑癌基因 p1 6在胰腺癌的发生、发展过程中起一定作用 ;P1 6蛋白的表达率与胰腺癌的恶性程度呈负相关 ;P1 6蛋白的表达率与病人术后生存时间呈正相关 ;P1 6蛋白的表达率与病人的年龄、性别、肿瘤大小、是否有淋巴结转移无关
Objective : To study the expression and significance of tumor suppressor gene p1 6 in pancreatic cancer. Methods: The expression of P16 protein in 28 cases of pancreatic adenocarcinoma and 6 cases of normal pancreas was detected by immunohistochemical LSAB method with p16 polyclonal antibody. RESULTS : 1 There was significant difference between the expression rate of P16 in pancreatic adenocarcinoma and the expression rate of P16 protein in pancreatic adenocarcinoma (P < 0.05). 2 There was no correlation between the expression rate of P16 in pancreatic adenocarcinoma and the patient’s age, gender, and tumor size (P>0.05). 3 The expression rate of P1 6 protein in pancreatic adenocarcinoma with different degree of differentiation was significantly different (P<0.05). 4 The expression rate of P1 6 protein in pancreatic adenocarcinoma was not statistically significant between the two groups (P>0.05). 5 There was a positive correlation between the expression rate of P1 6 protein in pancreatic adenocarcinoma and the survival time of patients (P<0.05). Conclusion: The tumor suppressor gene p16 plays a role in the occurrence and development of pancreatic cancer. The expression rate of P16 protein is negatively correlated with the degree of malignancy of pancreatic cancer. The expression rate of P16 protein is positively correlated with the survival time of patients. The expression rate of P16 protein is independent of the patient’s age, gender, tumor size, and whether there is lymph node metastasis.