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目的:观察苦豆子总碱(total alkali sophora alopecuroids L,TASa)对大鼠胃窦环形肌条的作用及机制。方法:采用离体恒温灌流浴槽,记录胃窦环形肌条收缩波的振幅、持续时间、收缩面积和频率,给予TASa(5、10、20、40、80mg/L)观察其量效关系(r);将标本分为对照组(NS)、TASa、TASa+阿托品(10-6mg/L)、TASa+酚妥拉明(10-6 mg/L)、TASa+苯海拉明(10-6 mg/L)、TASa+消炎痛(10-6 mg/L)和TASa+异搏定(10-7 mg/L)七组探讨TA-Sa收缩肌条的作用机制。结果:TASa能增强肌条收缩的振幅、持续时间和收缩面积,并有量效依赖性(振幅:r=0.96,t=23.72,P<0.01;持续时间:r=0.95,t=32.55,P<0.01;收缩面积:r=0.98,t=23.68,P<0.01),但对其收缩频率(NS:3.58±0.15,TASa:3.72±0.21,P>0.05)无影响;异搏定能明显抑制TASa收缩肌条的作用,而阿托品、酚妥拉明、苯海拉明和消炎痛无影响。结论:TASa可能是通过激活Ca2+通道,升高胞浆内Ca2+而加强肌条的收缩。
Objective: To observe the effect and mechanism of total alkali sophora alopecuroids L (TASa) on the gastric antral muscle strips in rats. Methods: The amplitude, duration, contractile area and frequency of contractile waves of gastric antral myenterium were recorded with a thermostatic perfusion bath in vitro. The dose-response relationship was observed by TASa (5, 10, 20, 40 and 80 mg / L) ) Were divided into control group (NS), TASa, TASa + atropine (10-6 mg / L), TASa + phentolamine (10-6 mg / L), TASa + diphenhydramine ), TASa + indomethacin (10-6 mg / L) and TASa + verapamil (10-7 mg / L). Results: TASa increased the amplitude, duration and contractile area of contracted muscle strips with dose-dependent dependence (amplitude: r = 0.96, t = 23.72, P <0.01; duration: r = 0.95, t = 32.55, P (NS: 3.58 ± 0.15, TASa: 3.72 ± 0.21, P> 0.05); Verapamil was significantly inhibited (P <0.01); Contraction area: r = 0.98, t = 23.68, TASa contractile muscle strips, and atropine, phentolamine, diphenhydramine and indomethacin no effect. Conclusion: TASa may enhance the contractions of muscle strips by activating Ca2 + channels and increasing intracellular Ca2 +.