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目的探讨胡桃醌对宫颈癌Caski细胞增殖影响及其促凋亡作用。方法选取处于对数生长期的宫颈癌Caski细胞,将其分为对照组和不同剂量胡桃醌组(20、40、60、80及100μmol/L)。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法观察胡桃醌对宫颈癌Caski细胞的增殖抑制作用,并计算出半数抑制浓度(IC50),依据IC50确定胡桃醌的有效浓度;在光学显微镜下观察细胞形态学变化;Hoechst 33258染色观察细胞核形态,流式细胞术测定胡桃醌对Caski细胞凋亡影响。结果 MTT结果显示,与对照组比较,胡桃醌40、60、80、100μmol/L组宫颈癌Caski细胞增殖[OD值分别为(0.65±0.11)、(0.53±0.14)、(0.40±0.11)和(0.31±0.05)]明显受到抑制,差异均有统计学意义(P<0.05);Hoechst 33258染色显示,40μmol/L胡桃醌培养12 h可明显导致细胞核浓缩;流式细胞仪检测表明,对照组Caski细胞早期凋亡率为(2.86±0.47)%,经40μmol/L的胡桃醌处理细胞12 h,早期凋亡率增加至(15.47±2.56)%(P<0.05)。结论不同浓度的胡桃醌可以抑制Caski细胞增殖,同时可诱导Caski细胞凋亡。
Objective To investigate the effect of juglone on the proliferation of cervical cancer Caski cells and its role in promoting apoptosis. Methods Caski cells in logarithmic growth phase were selected and divided into control group and different doses of juglone (20, 40, 60, 80 and 100 μmol / L). The inhibitory effect of juglone on the proliferation of cervical cancer Caski cells was observed by methyl thiazolyl tetrazolium bromide (MTT) method and the half inhibition (IC50). The effective concentration of juglone was determined by IC50. Morphological changes of cells were observed under a light microscope. The morphology of nuclei was observed by Hoechst 33258 staining. The apoptosis of Caski cells was assayed by flow cytometry. Results The results of MTT showed that compared with the control group, the proliferation of cervical cancer Caski cells in 40, 60, 80 and 100 μmol / L juglone groups were (0.65 ± 0.11), (0.53 ± 0.14), (0.40 ± 0.11) and (0.31 ± 0.05)] was significantly inhibited (P <0.05). Hoechst 33258 staining showed that 40μmol / L juglone could significantly induce nuclear condensation after cultured for 12 hours. Flow cytometry showed that the control group The early apoptosis rate of Caski cells was (2.86 ± 0.47)%. The apoptosis rate of Caski cells increased to (15.47 ± 2.56)% (P <0.05) after treated with 40μmol / L juglone for 12 hours. Conclusion Different concentrations of juglone can inhibit the proliferation of Caski cells and induce the apoptosis of Caski cells.