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目的探索应用多细胞系微量细胞培养法进行病脑脑脊液病毒分离,以建立简便、快速的病毒初筛方法。方法采集病毒性脑炎病例脑脊液46份,应用4种细胞系在96孔细胞培养板进行分离病毒,所得培养物用肠道病毒通用引物RT-PCR扩增,进行初步鉴定。结果 46份标本分离病毒11株,其中3株应用肠道病毒通用引物扩增出预期片段。结论多细胞系微量细胞培养法具有快速、便捷的特点,是一种较好的病毒初筛方法。
Objective To explore the separation of virus from cerebrospinal fluid of cerebrospinal fluid by using multi-cell line micro-cell culture method to establish a simple and rapid virus screening method. Methods A total of 46 cerebrospinal fluid samples of patients with viral encephalitis were collected. Four kinds of cell lines were used to isolate the virus in 96-well cell culture plates. The resulting culture was amplified by RT-PCR using universal primers for enterovirus. Results Of the 46 specimens, 11 strains were isolated, of which 3 strains were amplified using the universal primers of enterovirus. Conclusion Multi-cell micro-cell culture method has the characteristics of fast and convenient, and is a good screening method of virus.