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目的建立超高效液相色谱-三重四级杆/线性离子阱质谱(UPLC-QTRAP-MS/MS)同时测定玄参不同产地及商品药材中环烯醚萜苷、苯丙素苷和有机酸类12种指标成分的方法。方法采用BDS HYPERSIL C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速为1.0 m L·min~(-1),柱温为35℃,负离子多反应监测(MRM)模式测定。结果 12种指标成分在一定浓度范围内线性关系良好,相关系数均大于0.999 4,平均加样回收率为99.59%~101.24%,相对标准偏差在0.93%~1.60%之间。结论该方法准确、可靠,为玄参药材内在质量的综合评价和全面控制提供可靠的检测方法。
OBJECTIVE To establish a method for the simultaneous determination of iridoid glycosides, phenylpropanoid glycosides and organic acids in different medicinal herbs of Radix Hyssopiae by ultra performance liquid chromatography-triple quadrupole / linear ion trap mass spectrometry (UPLC-QTRAP-MS / MS) Method of index components. Methods BDS HYPERSIL C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and water with gradient elution at a flow rate of 1.0 m L · min -1. The column temperature was 35 ℃. Response monitoring (MRM) mode determination. Results The linearity of 12 index components was good in a certain range of concentration. The correlation coefficients were all above 0.999 4. The average recoveries were 99.59% ~ 101.24% with relative standard deviations between 0.93% ~ 1.60%. Conclusion The method is accurate and reliable, and provides a reliable method for comprehensive evaluation and comprehensive control of the intrinsic quality of Radix Scrophulariaceae.