论文部分内容阅读
背景:骨髓多能成体祖细胞具有比骨髓间充质干细胞更强的分化潜能和扩增能力并具有胚胎干细胞样的多向分化潜能,但分化为成软骨样细胞的研究目前少见报道。目的:观察成人骨髓成体多能祖细胞的体外培养条件、生物学特性及其向成软骨样细胞分化的可能性。方法:观察体外培养成人骨髓成体多能祖细胞的形态、生长情况,并鉴定;以分化培养基诱导成人骨髓成体多能祖细胞向无软骨细胞分化。结果与结论: 光学显微镜下观察培养的成人骨髓多能成体祖细胞体积偏大,长多角型,数个细长伪足,细胞核大,细胞质丰富,细胞增殖能力旺盛;可见转录因子 4、阶段特异性胚胎表面抗原 1表达,未见 T 细胞表面黏附分子 CD44,CD45,CD133 和主要组织相容性抗原系统Ⅱ的表达;诱导分化后,分化细胞经甲苯胺蓝染色后呈异染性,可表达Ⅱ型胶原。证实,实验成功体外培养了成人骨髓多能成体祖细胞,骨髓多能成体祖细胞在一定诱导条件下具有向成软骨样细胞分化的潜能。
BACKGROUND: Bone marrow multipotent adult progenitor cells have stronger differentiation and expansion abilities than bone marrow mesenchymal stem cells and have embryonic stem cell-like multipotentiality. However, the studies on differentiation into chondrogenic cells are rarely reported at present. OBJECTIVE: To observe the in vitro culture conditions, biological characteristics and the possibility of differentiating into adult chondrogenic cells in adult human bone marrow multipotent progenitor cells. Methods: The morphology and growth of adult adult human bone marrow multipotent progenitor cells were observed and identified. Differentiation medium was used to induce adult human bone marrow multipotent progenitor cells to differentiate into chondrocytes. RESULTS AND CONCLUSION: The adult BMSCs cultured in vitro showed large size, long polygons and several pseudopods with large nucleus, abundant cytoplasm and strong proliferative capacity. The transcription factor 4, stage-specific No expression of T cell surface adhesion molecule CD44, CD45, CD133 and major histocompatibility antigen system Ⅱ was observed. The differentiated cells were differentially expressed after toluidine blue staining and were expressed Type II collagen. Confirmed that the experiment successfully cultured in vitro adult human bone marrow multipotent progenitor cells, bone marrow multipotent progenitor cells under certain inducing conditions have the potential to differentiate into chondrogenic cells.