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用培养的鸡胚脑神经细胞研究了１．０２和２．０４ｍｍｏｌ·Ｌ－１氯丙烯作用２４ｈ后细胞内Ｃａ２＋，游离钙调蛋白（ＣａＭ）和环腺苷酸（ｃＡＭＰ）含量及钙／钙调蛋白依赖性蛋白激酶Ⅱ（Ｃａ２＋／ＣａＭ－ＰＫⅡ）活性的改变．结果显示，随着氯丙烯浓度的增加，细胞内Ｃａ２＋分别增加了４．２和６．２倍，ｃＡＭＰ含量增加了２２％和３９％，Ｃａ２＋／ＣａＭ－ＰＫⅡ活性增加了２．８和５．０倍（Ｐ＜０．０１）；而游离ＣａＭ含量则分别减少了５５％和６９％（Ｐ＜０．０１）．结果提示氯丙烯引起病理性神经轴浆内微管和神经微丝堆积与细胞内Ｃａ２＋增加有关．这可能由于细胞内Ｃａ２＋增加，激活ＣａＭ，继而激活Ｃａ２＋／ＣａＭ－ＰＫⅡ，催化微管相关蛋白２和ｔａｕ－蛋白磷酸化，抑制微管组装，使解聚的微管堆积在轴浆内．
The effects of 1.02 and 2.04mmol·L-1 allyl chloride on intracellular Ca2 +, free calmodulin (CaM) and cAMP and calcium / calcium Changes in the Activities of Amyloid-dependent Protein Kinase Ⅱ (Ca2 + / CaM-PKII). The results showed that the intracellular Ca2 + increased by 4.2 and 6.2 times, the cAMP content increased by 22% and 39%, and the activity of Ca2 + / CaM-PKⅡ increased by 2.8 and 5 with the increase of allyl chloride concentration. (P <0.01), while the content of free CaM decreased by 55% and 69% (P <0.01), respectively. The results suggest that the accumulation of microtubules and neurofilaments in alveolar nucleus induced by allyl chloride is related to the increase of intracellular Ca2 +. This may be due to intracellular Ca2 + increase, activate CaM, and then activate Ca2 + / CaM-PKⅡ, catalyze the microtubule-associated protein 2 and tau-protein phosphorylation, inhibition of microtubule assembly, the depolymerization of microtubules deposited in the axoplasm.