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AIM:To investigate the expression of effector proteasereceptor-1 (EPR-1),proliferative index ki-67 and apoptosisindex in patients with primary advanced gastricadenocarcinoma and to clarify the significance of EPR-1expression and its correlationship with the proliferationand apoptosis indexes.METHODS:Using immunohistochemical staining andterminal deoxynucleotidyl transferase mediated nick endlabelling (TUNEL) technique,we determined the expressionof EPR-1,proliferative index (Ki-67) and apoptotic index(AI) in 120 paraffin-embedded specimens of primaryadvanced gastric adenocarcinoma as well as lymph nodemetastasis and adjacent normal tissues.RESULTS:EPR-1 expression was distributed in thecytoplasm of normal gastric mycoderma,carcinoma cellsand smooth muscle cells.The positive rate of EPR-1expression in the primary gastric adenocarcinomas,invasiontumor node and lymph node metastasis was 65.83%,55.29%and 68%,respectively.While the positive rate in normalgastric mycoderma and smooth muscle cells was 46.7%and 53.3%,respectively.The average positive rate of ki-67in EPR-1-positive tumors was 7.00% which was significantlylower than that of 8.53% in EPR-1-negative tumors,butthe average AI in EPR-1-positive tumors was 1.25%,whichwas significantly higher than that of 1.00% observed inEPR-1-negative tumors.On the other hand,the averagepositive labeling index for Ki-67 (ki-67) in EPR-1-positivelymph node metastasis was 7.65%,which was significantlylower than that of 9.44% observed in EPR-1-negative lymphnode metastasis.However,the average AI in EPR-1-positivelymph node metastasis tumors was 0.99%,which wassignificantly higher than that of 0.67% observed in EPR-1-negative lymph node metastasis.CONCLUSION:The frequency of EPR-1 expression wassignificantly higher in primary gastric adenocarcinoma andin its lymph node metastasis than that in normal gastricmucosa.Expression of EPR-1 was significantly correlatedwith tumor histological subtypes and tumor differentiation.Weighted EPR-1 Score is positively correlated with apoptosis index,but is negatively related with proliferative index.Thus,Weighted EPR-1 Score and EPR-1 expression ingastric adenocarcinoma cells maybe a potential marker inclinical setting.
AIM: To investigate the expression of effector protease receptor-1 (EPR-1), proliferative index ki-67 and apoptosis index in patients with primary advanced gastric adenocarcinoma and to clarify the significance of EPR-1 expression and its correlationship with the proliferation and apoptosis indexes. METHODS: Using immunohistochemical staining and terminal deoxynucleotidyl transferase mediated nick endlabelling (TUNEL) technique, we determined the expression of EPR-1, proliferative index (Ki- 67) and apoptotic index (AI) in 120 paraffin- embedded specimens of primary gastric adenocarcinoma as well as lymph nodemetastasis and adjacent normal tissues .RESULTS: EPR-1 expression was distributed in thecytoplasm of normal gastric mycoderma, carcinoma cells and smooth muscle cells. The positive rate of EPR-1expression in the primary gastric adenocarcinomas, invasiontumor node and lymph node metastasis was 65.83%, 55.29 % and 68%, respectively.While the positive rate in normalgastric mycoderma and smooth muscle cells were 46.7% and 53.3% respectively.The average positive rate of ki-67in EPR-1-positive tumors was 7.00% which was significantlylower than that of 8.53% in EPR-1-negative tumors, but the average positive rate of ki- -positive tumors was 1.25%, which was significantly higher than that of 1.00% observed in EPR-1-negative tumors. On the other hand, the average positive labeling index for Ki-67 (ki-67) in EPR-1 positivelymph node metastasis was 7.65%, which was significantly lower than that of 9.44% observed in EPR-1-negative lymphnode metastasis. Despite, the average AI in EPR-1-positivemph node metastasis tumors was 0.99%, which wassignificantly higher than that of 0.67% observed in EPR -1-negative lymph node metastasis. CONCLUSION: The frequency of EPR-1 expression wassignificantly higher in primary gastric adenocarcinoma and in its lymph node metastasis than that in normal gastric mucosa. Expression of EPR-1 was predicted correlated with tumor histological subtypes and tumor differentiation. Weighted EPR-1Score is positively correlated with apoptosis index, but is negatively related to proliferative index. Thus, Weighted EPR-1 Score and EPR-1 expression ingastric adenocarcinoma cells maybe a potential marker inclinical setting.