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番木瓜环斑花叶是一种危险性的病毒病。用浸出法及2%磷钨酸负染的病叶汁标本,在电子显微镜下观察到线状颗粒,呈长而稍弯的线状构造,直径为10-15nm,平均为12.5nm,长度最长为1200nm,最短为200nm,长度在700-800nm的线状颗粒最多,占总数的69.3%。在病叶的超薄切片中,同样也观察到线状颗粒。这些线状颗粒多达几百个,平行排列,集中成束,位于靠近细胞壁的原生质中。健叶不论采用浸出法或超薄切片制备的标本,均未观察到与上述相同或相似的线状颗粒。所以,我们认为这些线状颗粒不是番木瓜的细胞成分,而是病原病毒的一种构造。此外,我们还观察到感染细胞内叶绿体变小、变形及其基质和淀粉粒减少的现象。
Papaya ring patch leaf is a dangerous virus disease. With leaching method and 2% phosphotungstic acid staining of diseased leaf juice specimens, the linear particles were observed under an electron microscope, a long and slightly curved linear structure, a diameter of 10-15nm, with an average of 12.5nm, the length of the most The length of 1200nm, the shortest 200nm, the length of 700-800nm linear particles, accounting for 69.3% of the total. In the thin sections of diseased leaves, linear particles were also observed. Up to several hundred of these linear particles, arranged in parallel, are concentrated in bundles, located in the protoplasm near the cell wall. No changes in the same or similar linear particles as above were observed in either of the healthy leaf samples prepared by either leaching or ultrathin sections. Therefore, we think these linear particles are not papaya cell components, but a structure of the pathogenic virus. In addition, we also observed that chloroplasts in infected cells became smaller, deformed and their matrix and starch grains reduced.