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[目的]比较CXC趋化配体10(CXCL10)和γ-干扰素(IFN-γ)在实验性矽肺大鼠肺组织中的表达,为矽肺发病机制的研究提供新的思路。[方法]12只健康雄性SD大鼠随机分为实验组和对照组,每组6只,采用非暴露气管灌注法分别对实验组和对照组大鼠染矽尘和灌注生理盐水,实验组大鼠一次性气管注入50mg/mL SiO2粉尘1mL,对照组大鼠注入同样体积的生理盐水。染尘40d后病理切片证实矽肺模型构建成功后,利用实时荧光定量聚合酶链反应和免疫组织化学法分别测定两组大鼠肺组织中CXCL10和IFN-γ基因及蛋白表达水平,比较二者的差异。[结果]实时荧光定量PCR显示两组CXCL10和IFN-γ基因表达水平没有差异。但是免疫组织化学结果发现,矽肺大鼠肺组织中CXCL10和IFN-γ蛋白表达水平均高于生理盐水对照组,差异具有统计学意义(P<0.05)。[结论]CXCL10和IFN-γ在纤维化实验组织中的表达高于正常肺组织,提示两个因子可能在矽肺纤维化过程中发挥着重要作用。
[Objective] To compare the expression of CXCL10 and IFN-γ in the lung tissue of experimental silicosis rats and to provide a new idea for the study on the pathogenesis of silicosis. [Methods] Twelve healthy male Sprague-Dawley rats were randomly divided into experimental group and control group, with 6 rats in each group. Rats in experimental group and control group were exposed to silica dust and saline, respectively, by non-exposed tracheal perfusion. One-time tracheal injection of 50mg / mL SiO2 dust 1mL, control group rats injected with the same volume of saline. Forty days later, the pathological results of the silicosis model confirmed that the expression of CXCL10 and IFN-γ mRNA and protein in the lung tissue of the two groups were detected by real-time fluorescence quantitative polymerase chain reaction and immunohistochemistry, respectively difference. [Result] Real-time fluorescence quantitative PCR showed no difference in CXCL10 and IFN-γ gene expression between the two groups. However, the results of immunohistochemistry showed that the expressions of CXCL10 and IFN-γ in lung tissue of silicotic rats were significantly higher than those in saline control group (P <0.05). [Conclusion] The expression of CXCL10 and IFN-γ in fibrosis experimental tissue is higher than that in normal lung tissue, suggesting that two factors may play an important role in the process of silicosis.