桑蚕丝蛋白的聚丙烯酰胺凝胶电泳研究——Ⅲ.绢丝蛋白的等电聚焦和双向电泳

• 作 者: 封云芳 陈碧源 刘华
• 机 构: 浙江丝绸工学院染化系,中山大学,浙江应用化学研究所,
• 出 处: 浙江丝绸工学院学报
• 发表日期: 1992年02期
• 关键词: 双向电泳 等电点 蚕丝蛋白 等电聚焦 绢丝蛋白 蛋白组成 大亚基 丝素蛋白 凝胶柱 蛋白质组成

本文用等电聚焦和双向电泳的方法,对桑蚕液状绢丝蛋白的组成成分进行了分析和研究。结果如下:丝胶的蛋白组成成分较中部丝素多而且分布宽,前者的等电点主要在pH为3.5～6,0的范围内;后者的等电点主要在pH5～7.0的区域内,是由一个分子量为2.8×10~5的大亚基和三个分子基为2.65×10~4(等电点为6.5、6.7和6.9)的小亚基为主要成分的多组分蛋白质;后部丝素在分子量和等电点分布方面均不同于中部丝素。

In this paper, the methods of isoelectric focusing and two-dimensional electrophoresis were used to analyze and study the composition of silkworm silkworm protein. The results are as follows: sericin protein composition than the middle of more than a wide distribution of silk, the former isoelectric point mainly in the range of pH 3.5 ~ 6,0; the latter mainly in the pH 5 ~ 7.0 area Is a multi-component protein composed of a large subunit with a molecular weight of 2.8 × 10 ~ 5 and three small subunits with molecular groups of 2.65 × 10 ~ 4 (isoelectric points of 6.5, 6.7 and 6.9) The posterior silk fibroin differs from the middle silk fibroin in terms of molecular weight and isoelectric point distribution.