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目的目前有报道认为淋巴细胞浸润与结肠癌(CRC)病理学变化相关,但CRC病人体内的淋巴细胞亚群各自的临床意义不清楚,本研究就此进行了探讨。方法我们使用了组织芯片和免疫组化技术检测67例CRC病人肿瘤组织中心区和边缘区CD4+和CD8+细胞密度,并分析其临床意义。结果我们发现,肿瘤浸润淋巴细胞亚群与病人的年龄、肿瘤大小、肿瘤的分期/分级不相关,且CD4+T细胞在不同分期/分级病人的肿瘤中心区和边缘区均无显著性差异。但是,与M1级病人相比,M0级病人肿瘤边缘区有更高的CD8+T细胞密度,而肿瘤中心区CD8+T细胞在两者间无差别。结论肿瘤边缘区高密度的CD8+T细胞与低肿瘤转移率相关。结果还提示对肿瘤浸润淋巴细胞亚群的分析有利于深入理解免疫系统对肿瘤的作用及其机制,且对开发相关干预措施提供了线索。
OBJECTIVE: It has been reported that lymphocytic infiltration is associated with the pathological changes of colon cancer (CRC), but the clinical significance of lymphocyte subsets in CRC patients is unclear. This study was carried out in this study. Methods We used tissue microarrays and immunohistochemical techniques to detect the density of CD4 + and CD8 + cells in the central and peripheral regions of 67 CRC patients and analyzed their clinical significance. Results We found that the infiltrating lymphocyte subsets were not related to the patient’s age, tumor size and tumor stage / grade. There was no significant difference in CD4 + T cells between the tumor center and marginal zone in different staging / grading patients. However, patients with M0 grade had higher CD8 + T cell density at the margins of the tumor compared with patients with M1 grade, while there was no difference between the CD8 + T cells at the tumor center. Conclusion The high density of CD8 + T cells in the marginal area of the tumor correlated with low tumor metastasis rate. The results also suggest that the analysis of tumor-infiltrating lymphocyte subsets is useful for understanding the effects of the immune system on the tumor and its mechanisms, and providing clues for the development of related interventions.