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血清多肽是癌症诊断信息的重要来源。近年来针对其复杂多样、丰度低、易降解的特点,多种多肽组技术得到快速发展。建立并优化了检测多肽标志物的免疫质谱方法,用于检测血清中的肿瘤多肽标志物。方法的线性范围为1～40pmol;检出限为0.5pmol;对5pmol合成肽5标准品平行检测3次的相对标准偏差(RSD)为9.5%。采用本方法检测出浓度为35.2nmol/L的血清多肽标志物。本方法适用于临床样本中低浓度标志物的检测研究,对于癌症的早期诊断具有重要意义。
Serum peptides are an important source of cancer diagnostic information. In recent years, for its complex and diverse, low abundance, easy degradation, a variety of peptide technology has been rapid development. An immuno-mass spectrometry method for detecting polypeptide markers was established and optimized for the detection of tumor peptide markers in serum. The linear range of the method was 1 ~ 40 pmol with a detection limit of 0.5 pmol. The relative standard deviation (RSD) of the 5 pmol synthetic peptide 5 standard was detected in triplicate for 9.5%. Using this method to detect the concentration of 35.2nmol / L serum polypeptide markers. The method is suitable for the detection of low concentration markers in clinical samples and is of great significance for the early diagnosis of cancer.