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试验比较了几种致病毒素诱变剂,以病叶冰冻提取液和改良 Fries 培养滤液的效果最理想,高粱米粒培养物的甲醇和蒸馏水提取液效果不理想。为防止致病毒素作用变化、制备诱变剂的小斑病菌最好用病叶保存。用改良 Fries 培养液培养小斑病菌每天振荡六次,每隔1小时一次、每次10分钟。所制备的培养滤液在5℃下可贮存六个月、用高压灭菌器灭菌30分钟及煮沸浓缩均不影响致病毒素的作用。评价致病毒素作用以比较抑制种子根生长的种苗萌发包法效果最好。愈伤组织无性系和试管苗抗病性的测试可用定量孢子的纸片法。
The test compared several mutagens of the virulence factors, with the frozen leaf extract and modified Fries culture filtrate the best results, sorghum grain culture methanol and distilled water extract results are not satisfactory. In order to prevent changes in the role of pathogenicity of the virus, the preparation of mutagen is best to use the diseased leaves. The S. hyoromellus was cultured six times a day with modified Fries medium, once every 10 hours for 1 hour. The prepared culture filtrate was stored at 5 ° C for six months, sterilized by an autoclave for 30 minutes, and concentrated by boiling without affecting the action of the pathogenicity toxin. Evaluation of the role of pathogenicity of the virus to inhibit the growth of seed germination seed germination package best. The callus clones and tube seedling disease resistance test can be used quantitative spore paper method.