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目的 研究日本血吸虫中国大陆株 2 3k Da膜蛋白 DNA疫苗基因枪免疫诱导 BAL B/ c小鼠产生的抗血吸虫感染作用。方法 6 0只雌性 BAL B/ c小鼠随机分为 6组 :pc DNA3.1组 (对照组 ) ,每只小鼠用基因枪经腹部皮肤免疫 pc DNA3.1质粒 DNA2次 ,共 2 μg;Sj C2 3基因枪 (gg)组 ,每鼠用基因枪免疫 pc DNA3.1- Sj C2 3质粒 DNA2 μg;Sj C2 3肌注 (im)组 ,每鼠肌注 10 0 μg pc DNA3.1- Sj C2 3质粒 DNA;Sj C2 3/ Cp G(gg)组 ,每鼠用基因枪免疫 pc DNA3.1- Sj C2 3/ Cp G质粒 DNA2 μg;Sj C2 3/Cp G(im)组 ,每鼠肌注 10 0 μg pc DNA3.1- Sj C2 3/ Cp G质粒 DNA;联合免疫组 ,每只小鼠先肌注 10 0μg pc DNA3.1- Sj C2 3/ Cp G质粒 DNA,第 2天用基因枪免疫 2 μg。各组小鼠隔 2周加强免疫 1次 ,共 3次。末次免疫后 4周每鼠经腹部皮肤攻击感染 (45± 1)条日本血吸虫尾蚴 ,4 5 d后剖杀 ,计数成虫及肝脏虫卵数。首次免疫前 2天及感染前 2天经尾静脉采血检测 Ig G抗体水平及抗体亚类 Ig G1 、Ig G2 a。末次免疫后 3周检测脾细胞经 Con A和 r Sj C2 3- HD刺激后培养上清中鼠 IL- 2、IL- 4和 IFN- γ的水平。结果 Sj C2 3(gg)组、Sj C2 3/ Cp G(gg)组及联合免疫组小鼠所检成虫数均低于对照组 (P均 <0 .0 1) ,减虫率
Objective To study the anti-schistosome infection induced by gene gun immunization in BALB / c mice induced by DNA vaccine of 23 kDa membrane protein of Schistosoma japonicum. Methods Sixty female BALB / c mice were randomly divided into 6 groups: pcDNA3.1 group (control group). Each mouse was immunized with pcDNA3.1 plasmid DNA twice with 2g of gene gun for 2 times. Sj C2 3 gene gun (gg) group, each mouse was immunized with gene gun pcDNA3.1-Sj C2 3 plasmid DNA 2 μg; Sj C2 3 im group, each mouse intramuscular injection of 10 0 pcDNA3.1- Sj C2 3 / Cp G (img) group, Sj C2 3 / Cp G (gg) group, pcDNA3.1-Sj C2 3 / The mice were intramuscularly injected with 10 μg pc DNA3.1-Sj C2 3 / Cp G plasmid DNA, and each group was injected intraperitoneally with 10 μg pcDNA3.1-Sj C2 3 / Cp G plasmid DNA at the second day 2 μg were immunized with a gene gun. Each group of mice every 2 weeks to strengthen the immune 1, a total of 3 times. Four weeks after the last immunization, mice (45 ± 1) were challenged with cercariae of Schistosoma japonicum by abdominal skin challenge and killed after 45 days. The numbers of adult and liver eggs were counted. Ig G antibody levels and antibody subclasses Ig G1 and Ig G2 a were measured by tail vein blood sampling 2 days before the first immunization and 2 days before infection. Three weeks after the last immunization, the levels of IL-2, IL-4 and IFN-γ in the culture supernatant were detected after spleen cells were stimulated with Con A and r Sj C2 3- HD. Results The numbers of adult worms in Sj C2 3 (gg) group, Sj C2 3 / CpG (gg) group and combined immunization group were lower than those in control group (all P <0.01). The worm reduction rate