Preparation of dextran immunological magnetic nanoparticles and their application to combined target

• 作 者: 李民勤 徐慧显 左榘 姬昂 何炳林 庞雁 黄建英 牛瑞芳
• 机 构: Institute of Polymer Chemistry, State Key Laboratory of Functional Polymer Materials for Adsorption
• 出 处: Science in China,Ser.B
• 发表日期: 1996年06期
• 关键词: dextran targeting immunological Dextran hydroxide retention ammonium mammary sal

Superparamagnetic dextran nanoparticles were prepared by coprecipitation. Effects of concentration of dextran, amount of ironic salts, stirring speed, concentration of ammonium hydroxide and mole ratio of Fe3+/Fe2+ on the effective diameter of dextran magnetic nanopartides (DMNP) were studied. Dextran immunological magnetic nanoparticles (DIMNP) were formed by the reaction of the monoclonal anti-human mammary cancer antibody with DMNP oxidized by sodium periodate, and the properties of magnetic susceptibility, shape and retention of antibody activity were investigated. The in vitro cancer antigen binding ability of DIMNP was demonstrated by radioimmunoassay, and the in vivo magnetic localization and antibody targeting ability of radiolabeled DIMNP were studied.

Superparamagnetic dextran nanoparticles were prepared by coprecipitation. Effects of concentration of dextran, amount of ironic salts, stirring speed, concentration of ammonium hydroxide and mole ratio of Fe3 + / Fe2 + on the effective diameter of dextran magnetic nanopartides (DMNP) were studied. Dextran immunological magnetic The in vitro cancer antigen binding ability of DIMNP (nanoparticles) was demonstrated by radioimmunoassay, and the in vivo magnetic localization and antibody targeting ability of radiolabeled DIMNP were studied.