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目的建立超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)测定酒、牡蛎粉、咖啡中那非类物质含量的分析方法。方法采用Waters HSS T3(2.1 mm×100 mm,1.8μm)反相色谱柱,以0.1%甲酸水和0.1%甲酸乙腈为流动相进行梯度洗脱,经质谱多反应监测(multiple reaction monitor,MRM)法进行检测。结果在10~200 ng/mL的浓度范围内,去甲基他达拉非和硫代西地那非均表现出良好的线性关系,2种化合物的检出限分别为50 ng/g和50 ng/mL,定量限分别为100 ng/g和100 ng/mL。结论该方法操作简便,结果可靠,灵敏度高,可用于食品中去甲基他达拉非和硫代西地那非含量的快速测定。
OBJECTIVE To establish a method for the determination of non-class substances in wine, oyster meal and coffee by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS). Methods The mobile phase of Waters HSS T3 (2.1 mm × 100 mm, 1.8 μm) was used to elute gradient elution with 0.1% formic acid and 0.1% formic acid as mobile phase. Multiple reaction monitor (MRM) Law for testing. Results In the concentration range of 10 ~ 200 ng / mL, both desmethyltadalafil and thio-sildenafil exhibited good linearity with the detection limits of 50 ng / g and 50 ng / mL, with a limit of quantification of 100 ng / g and 100 ng / mL, respectively. Conclusion The method is simple, reliable, sensitive and can be used for the rapid determination of demethyl-tadalafil and thia-sildenafil in food.