论文部分内容阅读
目的优化大孔吸附树脂分离纯化葡萄叶黄酮类化合物的工艺条件。方法采用分光光度法,以芦丁为标准品,采用硝酸铝显色法测定总黄酮含量;以葡萄叶总黄酮的含量、吸附量和解吸率为考察指标,通过静态及动态实验,筛选出理想的吸附树脂,并通过单因素试验确定了葡萄叶总黄酮富集分离的优选操作条件。结果 D-101树脂是吸附葡萄叶提取液中总黄酮较理想的树脂,对其优化的工艺参数为:最佳吸附p H值:p H=3,洗脱剂浓度:70%的乙醇,最佳上样流速:0.8 m L/min,最佳上样液浓度:65.65 mg/L,最佳解吸流速:0.8m L/min。结论 D-101树脂对葡萄叶总黄酮的吸附量大,解吸容易,环境友好,确定的吸附与洗脱条件简单可行,此工艺具有良好的产业化前景。
Objective To optimize the conditions for the separation and purification of flavonoids from grape leaves by macroporous adsorption resin. Methods The content of total flavonoids was determined by spectrophotometry with rutin as standard and aluminum nitrate colorimetry. The content of total flavonoids, adsorption capacity and desorption rate of grape leaves were taken as the indexes. Static and dynamic experiments were carried out to select the ideal The optimum operating conditions for the enrichment and separation of total flavonoids from grape leaves were determined by single factor experiments. Results D-101 resin was the ideal resin for the adsorption of total flavonoids in grape leaf extract. The optimized parameters were as follows: p H = 3, ethanol concentration 70% Optimal sample flow rate: 0.8 m L / min, the best sample concentration: 65.65 mg / L, the best desorption flow rate: 0.8m L / min. Conclusions D-101 resin has high adsorption capacity for total flavonoids from grape leaves, easy desorption and environment-friendly. It is simple and feasible to determine the adsorption and elution conditions. The technology has good industrialization prospects.