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在小麦花药离体培养中,由愈伤组织分化的绿苗往往只长茎叶不长根。据统计,45个杂交组合的157株中就有139株幼苗无根(88.5％),移栽难以存活,从而大大限制纯合二倍体植株的获得,给单倍体育种工作带来困难。为此,我们在原来工作基础上着重对生长素种类、浓度及植物提取液的促根效果进行研究。经过试验,初步筛选出发根早、根数多、适应性较广的R_3生根培养基。现将试验资料整理如下: 材料和方法供试材料系45个杂交组合F_1花药分化出的无根幼苗。基本培养基为White(怀特)和N_6两
In vitro culture of wheat anthers, callus differentiation of green seedlings are often long stems and leaves do not grow roots. According to statistics, 139 out of 157 out of 45 hybrids have no roots (88.5%), which makes it hard to survive after transplanting, which greatly limits the obtainment of homozygous diploid plants and poses difficulties for haploid breeding. To this end, we focused on the original work focused on the type of auxin, concentration and plant extracts to promote rooting. After testing, preliminary screening root early, the number of roots, adaptability of R_3 rooting medium. The test data are now organized as follows: Materials and methods The test materials were 45 hybrid combinations F_1 anther differentiation of rootless seedlings. The basic medium for White (White) and N_6 two