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利用数量性状基因位点(QTLs)进行分子作图的主要难度是:(1)建立一个从农艺性状上可接受的作图群体,通过这个群体来测定不含非正常分离的微小连锁效应。(2)建立有效的分子标记系,用以测定众多的多态标记基因。本研究的目标是利用基因重组近交系(RI)有效地创
The main difficulty in molecular mapping with QTLs is to: (1) establish a mapping population that is acceptable from agronomic traits and use this population to determine the micro-chain effect without abnormal segregation. (2) To establish an effective molecular marker system for the determination of a large number of polymorphic marker genes. The goal of this study was to efficiently genotype the gene using recombinant inbred lines (RIs)