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用现有的OsCCR基因片段筛选水稻核DNA文库,获得了长为3045 bp的核DNA.它包含4个内含子和5个外显子,推测的可读框长为337个氨基酸.OsCCR与其他植物中同类酶在氨基酸水平上有70％的相似性和30％的相同性,含有保守的辅酶Ⅱ(NADP)结合位点及可能为该酶催化位点的特征序列.Northern杂交证实,该基因产物广泛存在于水稻根、茎和叶等各组织中,在茎中表达量较高.组织原位杂交结果表明,OsCCR基因在维管束及新生侧根中有强烈的表达信号,与该基因编码的酶的木质素合成特性相吻合.OsCCR基因的克隆、组织定位及生物化学方面的研究将有助于深入了解木质素形成的分子机理,并为通过遗传工程的手段改善植物的机械性能以获得低木质素纸浆原料打下基础.
Using the existing OsCCR gene fragment to screen the rice nuclear DNA library, a nuclear DNA of 3045 bp was obtained, containing 4 introns and 5 exons, and the deduced open reading frame length was 337 amino acids.OsCCR and Other plants have 70% similarity and 30% identity at the amino acid level, and contain the conserved Coenzyme Ⅱ (NADP) binding site and the characteristic sequence that may be the catalytic site of the enzyme. Northern hybridization confirmed that the The gene products widely existed in the tissues of roots, stems and leaves of rice, and were highly expressed in stems. The results of in situ hybridization showed that OsCCR gene strongly expressed in vascular bundles and newborn lateral roots, Of the enzyme lignin synthesis characteristics consistent.OsCCR gene cloning, tissue localization and biochemical aspects of the study will help to understand the molecular mechanism of lignin formation and genetic engineering tools to improve the mechanical properties of plants to obtain Low lignin pulp raw material laid the foundation.