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目的观察菩人丹超微粉(Pu Ren Dan Superfine Powder,PRD)对2型糖尿病(Type 2 Diabetes Mellitus,T2DM)大血管病变大鼠骨骼肌组织胰岛素受体底物-1(insulinreceptor substrate-1,IRS-1)mRNA及葡萄糖转运蛋白4(glucose transporter4 GLUT4)mRNA表达的影响,探讨PRD调控骨骼肌葡萄糖转运的分子机制。方法以Wistar大鼠造T2DM模型大鼠,造模成功的T2DM大鼠按血糖结合体质量分层随机分为5组:模型组、罗格列酮组、PRD低、中、高剂量(0.885g/kg;1.770g/kg;3.540g/kg)治疗组,另取10只大鼠作为正常对照组。各组动物每日灌胃给药1次,正常对照组和T2DM模型组灌胃等剂量蒸馏水,连续给药4周。实验期间除正常对照组给予普通饲料外,其余各组均给予高脂高热量饲料。采用反转录PCR(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)技术和凝胶电泳成像分析方法,观察T2DM大血管病变大鼠骨骼肌组织IRS-1、GLUT4mRNA表达。结果 T2DM大血管病变大鼠骨骼肌组织IRS-1、GLUT4mRNA表达与正常对照组比较明显降低(P<0.01);PRD治疗组均能提高T2DM大鼠骨骼肌组织IRS-1、GLUT4mRNA的表达(P<0.05,P<0.01)。结论 PRD可提高T2DM大血管病变大鼠骨骼肌IRS-1mRNA及GLUT-4mRNA的表达水平。
Objective To observe the effect of Pu Ren Dan Superfine Powder (PRD) on insulin receptor substrate-1 (IRS) in skeletal muscle of rats with type 2 diabetes mellitus (T2DM) -1) mRNA and glucose transporter4 (GLUT4) mRNA expression, and explore the molecular mechanism of PRD regulating glucose transport in skeletal muscle. Methods T2DM model rats were established in Wistar rats. The successful T2DM rats were randomly divided into five groups according to their blood glucose and body weight: model group, rosiglitazone group, PRD low, medium and high dose (0.885g /kg;1.770g/kg; 3.540g/kg) treatment group, another 10 rats as a normal control group. Each group of animals given intragastrically once a day, normal control group and T2DM model group, intragastric administration of distilled water, continuous administration for 4 weeks. Except the normal control group were given normal feed during the experiment, the rest of the groups were given high-fat and high-calorie feed. Reverse transcription-polymerase chain reaction (RT-PCR) and gel electrophoresis were used to observe the mRNA expression of IRS-1 and GLUT4 in skeletal muscle tissue of rats with T2DM macroangiopathy. Results The expression of IRS-1 and GLUT4 mRNA in skeletal muscle of rats with T2DM macroangiopathy was significantly lower than that of the normal control group (P <0.01). The expression of IRS-1 and GLUT4 mRNA in skeletal muscle of T2DM rats increased <0.05, P <0.01). Conclusions PRD can increase the expression of IRS-1mRNA and GLUT-4mRNA in skeletal muscle of rats with T2DM macroangiopathy.