论文部分内容阅读
【目的】研究降雨条件对浴场细菌总数和优势菌群组成的影响。【方法】2014年8月强降雨前后采集秦皇岛西浴场3个站位的海水样品,采用荧光显微镜计数法和平板计数法分别对细菌总数和可培养细菌总数进行计数;对群落结构组成进行分析,并对可培养细菌进行鉴定。【结果】雨前3个站位细菌总数和可培养细菌总数平均值分别为5.6×10~9 CFU/L和8.3×10~7 CFU/L,雨后分别为9.2×109 CFU/L和2.1×10~8 CFU/L。在可培养菌群中,变形菌门(Proteobacteria,雨前占80%,雨后占73%)是主要的微生物类群,其次为拟杆菌门(Bacteroides,雨前占12%,雨后占13%)、厚壁菌门(Firmicutes,雨前占7%,雨后占11%)等;肠杆菌属(Enterobacter spp.,21株)、海杆菌属(Marinobacter spp.,13株)、弓形菌属(Arcobacter spp.,13株)、假单胞菌属(Pseudomonas spp.,10株)、芽孢杆菌属(Bacillus spp.,10株)和弧菌属(Vibrio spp.,6株)为雨前可培养细菌优势属,而雨后可培养细菌优势属为肠杆菌属(22株)、海杆菌属(21株)、芽孢杆菌属(14株)、不动杆菌属(Acinetobacter spp.,11株)、假单胞菌属(9株)和弓形菌属(5株)等。【结论】降雨对细菌总数有显著的影响,同时降雨后浴场微生物群落结构发生了改变。
【Objective】 The objective of this study was to investigate the effects of rainfall on the total number of bacteria in the bathing beach and the dominant microflora. 【Method】 Seawater samples collected at three stations in Qinhuangdao West Bathing Area before and after heavy rainfall in August 2014 were collected. The total number of bacteria and the total number of culturable bacteria were counted respectively by fluorescence microscopy and plate count method. The composition of community structure was analyzed, Bacteria can be identified. 【Result】 The results showed that the average numbers of bacteria and cultivable bacteria in the three stations before rain were 5.6 × 10 ~ 9 CFU / L and 8.3 × 10 ~ 7 CFU / L, respectively, and 9.2 × 109 CFU / L and 2.1 × 10 ~ 8 CFU / L. Proteobacteria (80% before rain and 73% after rain) were the main microbial groups in the culturable flora, followed by Bacteroides (12% before rain and 13% after rain) ), Firmicutes (7% before rain and 11% after rain), and the like; Enterobacter spp., 21 strains, Marinobacter spp., 13 strains, (13 strains), Pseudomonas spp. (10 strains), Bacillus spp. (10 strains) and Vibrio spp. (6 strains) Bacterial genus is cultivated, while bacterium which can cultivate after rain is of the genus Enterobacter (22 strains), Schisandra (21 strains), Bacillus (14 strains), Acinetobacter spp. (11 strains) , Pseudomonas (9 strains) and Arcobacter (5 strains) and so on. 【Conclusion】 Rainfall has a significant effect on the total number of bacteria, and the microbial community structure has changed after rainfall.