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[目的]探讨miR-214对肺腺癌细胞PC9增殖和凋亡的影响。[方法]培养人肺腺癌细胞株PC9(EGFR基因19外显子缺失,对EGFR-TKI吉非替尼敏感),将miR-214类似物(miR-214 mimic)及对照物(scramble)转染至PC9细胞中,在不同的时间点(24、48、72h)使用MTT检测细胞的增殖;转染细胞后,加入吉非替尼共培养48h后,使用流式细胞仪检测细胞凋亡;同时建立PC9荷瘤小鼠,观察荷瘤小鼠瘤体的生长曲线,及尾静脉注射miR-214抑制剂(antigomiR-214)后观察荷瘤小鼠瘤体的生长。[结果 ]在PC9细胞中,miR-214 mimic被转染入细胞后,48h及72h后细胞增殖率分别增加了27.9%和18.7%,相比转让了对照组的细胞差异均有统计学意义(P均<0.05)。吉非替尼可以诱导PC9细胞凋亡,但转染miR-214mimic后细胞凋亡率由86.5%下降为28.5%。在荷瘤小鼠中抑制miR-214后,小鼠瘤体明显缩小(P<0.05)。[结论]miR-214可促进肺腺癌细胞增殖及抗凋亡,可能可作为肺腺癌治疗的新靶点。
[Objective] To investigate the effect of miR-214 on the proliferation and apoptosis of lung adenocarcinoma cell line PC9. [Methods] Human lung adenocarcinoma cell line PC9 (EGFR gene exon 19 deletion, sensitive to EGFR-TKI gefitinib) was cultured and transfected with miR-214 mimic and scramble The cells were transfected into PC9 cells, and the proliferation of PC9 cells was detected by MTT at different time points (24,48,72h). After transfected with Gefitinib for 48h, the apoptosis was detected by flow cytometry. At the same time, PC9 tumor-bearing mice were established to observe the growth curve of tumor-bearing mice, and the growth of tumor-bearing mice was observed after the anti-miR-214injection into tail vein. [Results] In PC9 cells, the proliferation rate of miR-214 mimic was increased by 27.9% and 18.7% respectively 48 h and 72 h after transfected into cells, which were significantly different from those of the control group P <0.05). Gefitinib induced apoptosis in PC9 cells, but apoptosis rate decreased from 86.5% to 28.5% after transfected with miR-214mimic. The inhibition of miR-214 in tumor-bearing mice significantly reduced tumor size (P <0.05). [Conclusion] miR-214 can promote the proliferation and anti-apoptosis of lung adenocarcinoma cells, which may be used as a new target for the treatment of lung adenocarcinoma.