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目的探讨肺癌患者血清及外周血单个核细胞中血小板源性生长因子-BB(PDGF-BB)的表达水平及其意义。方法采用酶联免疫吸附试验(ELISA)检测30名肺癌患者和20名正常对照者外周血血清中PDGF-BB的水平,用半定量逆转录-聚合酶链反应(RT-PCR)方法检测受试者外周血单个核细胞(PBMCs)中PDGF-B链mRNA的表达水平。结果肺癌患者和正常对照者血清PDGF-BB水平分别为(389.64±36.42)、(28.36±8.35)pg/ml,外周血单个核细胞PDGF-B链mRNA的表达水平分别为(0.9287±0.0942)、(0.2275±0.0384)灰度比值,比较差异有统计学的意义(P<0.01)。结论检测血清PDGF-BB及PBMCs中PDGF-B链mRNA表达水平可能对肺癌诊断具有一定临床指导意义。
Objective To investigate the expression of platelet-derived growth factor-BB (PDGF-BB) in serum and peripheral blood mononuclear cells of lung cancer patients and its significance. Methods The levels of PDGF-BB in peripheral blood of 30 lung cancer patients and 20 normal controls were detected by enzyme-linked immunosorbent assay (ELISA), and the levels of PDGF-BB were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) PDGF-B mRNA expression in peripheral blood mononuclear cells (PBMCs). Results The serum levels of PDGF-BB in peripheral blood mononuclear cells were (389.64 ± 36.42) and (28.36 ± 8.35) pg / ml respectively, and the levels of PDGF-B mRNA in peripheral blood mononuclear cells were (0.9287 ± 0.0942) (0.2275 ± 0.0384) gray ratio, the difference was statistically significant (P <0.01). Conclusion Detection of PDGF-B mRNA expression in serum PDGF-BB and PBMCs may be of clinical significance in the diagnosis of lung cancer.