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水稻雌配子的发育直接影响水稻的育性。由于雌配子败育的机理复杂,使得雌性不育的分子机理研究一直未取得突破性进展。本研究以EMS诱变获得的半不育突变体M21为研究材料,采用细胞学技术发现M21是由胚囊部分败育造成的。以M21//M21/N22回交群体BC1F1为作图群体全基因组分析,构建全长为2674.2 cM的遗传图谱,包含了189对SSR标记,平均遗传距离为14.1 cM;复合区间作图法(CIM)进行QTL定位,在第11号染色体检测到一个新的雌配子半不育QTL qPS-a,初定位在标记RM26761和RM26841之间,其贡献率为20.2%。本研究有助于进一步探究水稻雌性半不育的遗传机制。
The development of female gametes in rice directly affects the fertility of rice. Due to the complicated mechanism of female gametes abortion, the molecular mechanism of female sterility has not made any breakthrough. In this study, M21, a semi-sterile mutant obtained by EMS mutagenesis, was used as material for the study. Cytology was used to find that M21 was partially aborted by the embryo sac. A total of 2674.2 cM genetic map was constructed with BC21F1 of M21 // M21 / N22 backcross population as the mapping group, and 189 SSR markers were constructed with an average genetic distance of 14.1 cM. The composite interval mapping (CIM) ), A QTL for QSP of female genitalia was detected on chromosome 11. The initial QTL was located between markers RM26761 and RM26841, with a contribution rate of 20.2%. This study is helpful to further explore the genetic mechanism of rice male sterility.