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T 淋巴细胞在体外培养时,当受丝裂原(如植物血凝素、刀豆球旦 A)或其它特异性抗原刺激时可转化为幼稚淋巴细胞和淋巴母细胞,并进行有丝分裂,这种转化的淋巴细胞不仅在形态上出现幼稚型,而且有蛋白质和核酸的参加。基于这一原理,目前常用淋巴细胞转化试验来研究细胞免疫状态重要指标之一。目前一般常用的操作方法是:抽取静脉血加入肝素抗凝之小瓶内,将抗凝血加入含有小牛血清的培养液瓶内,再加 PHA 混匀至37℃培养,这样操作手续复杂,并容易污染。
When cultured in vitro, T lymphocytes can be converted into immature lymphocytes and lymphoblasts when stimulated by mitogen (such as phytohemagglutinin, concanavalin A) or other specific antigens, and undergo mitosis. The transformed lymphocytes not only appear naive in morphology, but also have the participation of proteins and nucleic acids. Based on this principle, lymphocyte transformation assays are currently used to study one of the important indicators of cellular immune status. At present, the commonly used method of operation is: withdrawing venous blood into a vial of heparin anticoagulation, adding anticoagulant blood to a culture bottle containing calf serum, and then adding PHA to mix it to 37°C for culture. This complicated operation procedure, and Easy to pollute.