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目的:观察蛇毒(SV)及其有效组分体内外的抗癌活性,为SV抗肿瘤新药的开发提供实验依据。方法:采用四甲基偶氮唑盐比色法(MTT法)筛查不同浓度(5～20mg/mL)SV及其7种分离组分对人结肠癌细胞株(HCT-8)、人肝癌细胞株(Bel-7402)、人口腔上皮癌细胞株(KB)、人乳腺癌细胞株(MCF-7)的影响;并用小鼠肝癌(H22)动物移植瘤作为实验动物模型,进一步观察SV及其组分Ⅳ(0.01～0.03mg/kg)对瘤重、脾重、外周血白细胞和体质量的影响。结果:SV、Ⅲ2和Ⅳ的肿瘤抑制作用较为明显,三者的浓度在20μg/mL时,对HCT-8、Bel-7402、KB以及MCF-7具有非常显著的抑制作用,对HCT-8的抑制率分别为84.37%、79.39%和85.78%;对Bel-7402的抑制率分别为89.64%、88.13%和92.08%;对KB的抑制率分别为90.08%、91.30%和93.93%;对MCF-7的抑制率分别为89.88%、89.49%和92.33%。腹腔注射10 d后,组分Ⅳ对H22有明显的抑制作用,SV组分Ⅳ3个浓度组的瘤重分别为(0.81±0.20)g、(0.70±0.18)g、(0.61±0.17)g,均明显低于对照组瘤重[(1.19±0.21)g,P<0.05],肿瘤生长抑制率(IR)分别为31.93%、41.17%和48.73%。Ⅳ各剂量组小鼠脾脏指数较对照组增加,白细胞数与对照组相比无明显变化。结论:SV及其7种分离组分均能有效抑制4种人肿瘤细胞株(HCT-8、Bel-7402、KB及MCF-7)的生长,不同的SV分离组分对同一肿瘤细胞抑制作用有差异;不同肿瘤细胞对同一SV分离组分的反应性也不同。SV分离组分Ⅳ对动物移植性肿瘤小鼠肝癌H22有良好的实验疗效。
Objective: To observe the anticancer activity of snake venom (SV) and its effective components in vitro and in vivo, and provide experimental evidence for the development of SV antitumor drug. Methods: MTT assay was used to screen human colon cancer cell line (HCT-8) and human hepatocellular carcinoma (HCC) with different concentrations of SV (5-20 mg / mL) (Bel-7402), human oral epithelial cancer cell line (KB), and human breast cancer cell line (MCF-7). The mouse hepatocellular carcinoma (H22) xenografts were used as experimental animal models, Its component IV (0.01 ~ 0.03mg / kg) on tumor weight, spleen weight, peripheral blood leukocytes and body weight. Results: The tumor inhibitory effects of SV, Ⅲ 2 and Ⅳ were obvious. The inhibitory effect of HCT-8, Bel-7402, KB and MCF-7 was significant at the concentration of 20μg / The inhibitory rates for Bel-7402 were 89.64%, 88.13% and 92.08%, respectively. The inhibitory rates for KB were 90.08%, 91.30% and 93.93%, respectively. The inhibitory rates were 84.37%, 79.39% and 85.78% 7 were 89.88%, 89.49% and 92.33% respectively. After intraperitoneal injection of 10 d, component IV had a significant inhibitory effect on H22. The tumor weight of the SV3 IV concentration group was (0.81 ± 0.20) g, (0.70 ± 0.18) g, (0.61 ± 0.17) g, Were significantly lower than those in the control group [(1.19 ± 0.21) g, P <0.05]. The tumor growth inhibition rates (IR) were 31.93%, 41.17% and 48.73%, respectively. Ⅳ dose group mice spleen index increased compared with the control group, the number of white blood cells compared with the control group no significant change. CONCLUSIONS: SV and its seven components can effectively inhibit the growth of four human tumor cell lines (HCT-8, Bel-7402, KB and MCF-7). The inhibitory effects of different SV fractions on the same tumor cell There are differences; different tumor cells on the same SV separation component reactivity is also different. SV separation of component IV on mice transplanted liver cancer H22 have a good experimental effect.